![]() “Similar resins can give different results for different target molecules, and with Bio-Rad’s EconoFit Column Packs our customers can easily perform resin scouting to identify the one which yields the best outcome for their chromatography application,” said Ertan Ozyamak, PhD, Bio-Rad Global Product Manager for lab-scale chromatography products. Columns are available in 1 ml and 5 ml sizes. The convenient, easy-to-use and disposable column format is fully compatible with Bio-Rad’s NGC Chromatography Systems and other commonly used chromatography systems. The new packs include mixed-mode, cation, and anion exchange resin columns, as well as a pack designed for His-tag protein purification. Molecules smaller than the col- umn’s exclusion limit will be retained by the column (see Specifications).Bio-Rad’s range of EconoFit Columns provides a wide selection of resins in a prepacked format, allowing customers to simply screen resins and select the optimal chemistry for different target molecules. Application of more or less than the recommended sample volume may decrease column per- formance.Īfter loading sample, centrifuge the column for 4 minutes at 1,000 x (g).įollowing centrifugation, the purified sample is now in either SSC or Tris buffer. Carefully apply the sample (20–75 µl) directly to the center of the column. Place the column in a clean 1.5 or 2.0 ml microcentrifuge tube. Discard the drained buffer then place the column back into the 2.0 ml tube.Ĭentrifuge for 2 minutes in a microcentrifuge at 1,000 x (g) (see Centrifugation Notes section) to remove the remain- ing packing buffer. Allow the excess packing buffer to drain by gravity to the top of the gel bed (about two minutes). If the column does not begin to flow, push the cap back on the column and then remove it again to start the flow. Snap off the tip and place the column in a 2.0 ml microcentrifuge tube (included). ![]() Invert the column sharply several times to resuspend the settled gel and remove any bubbles. PH 2–10, common aqueous buffers, formamide, dilute organic acids, alcohol, 20% (V/V) other chaotropic agents, detergents. Micro Bio-Spin columns, Bio-Gel P gel, and collection tubes are completely autoclavable at 121 ☌ for 30 minutes at pH 6.0–8.0. Microcentrifuge with a centrifugal force of 1,000 x (g). Volumes less than 20 µl may affect recoveryīio-Gel P-6 gel: 5 base pairs (nucleic acids) or 6,000 daltons (proteins,peptides)īio-Gel P-30 gel: 20 base pairs (nucleic acids) or 40,000 dal- tons (proteins, peptides)ĩ8% retention of unincorporated nucleotides at 20 µl 90% recovery of applied DNA at 20 µlġ00% retention of unincorporated nucleotides at 20 µl 95% recovery of applied DNA at 70 µl Nucleic acids, proteins, and peptides, 20–75 µl. Tris buffer (10 mM Tris-HCl, pH 7.4) with 0.02% sodium azide SSC buffer (150 mM sodium chloride, 17.5 mM sodium cit- rate, pH 7.0) with 0.02% sodium azide Micro Bio-Spin columns are suitable for use with 1.5 or 2.0 ml microcentrifuge tubes and are completely autoclavable.īio-Gel P-6 or P-30 polyacrylamide gel suspended in 1.0 ml of buffer ![]() This unique gel produces very effi- cient, non-interactive size separations. ![]() ![]() The columns are packed with special grades of Bio-GelĪcrylamide P-6 or P-30 gel matrices manufactured specifically for Bio-Rad spin columns. Desalt nucleic acids, proteins and peptides.Micro Bio-Spin chromatography columns are ready to use for rapid and efficient cleanup and purification of nucleic acids and proteins using a microcentrifuge. ![]()
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